DNA technology method plays an essential role in the pharmaceutical industry. With the help of this technology, vaccines, drugs, growth hormones, and therapeutic hormones have been made. DNA technology helps in the diagnosis of several diseases such as Huntington’s disease and sickle cell anemia. Gene therapy is also one of the most important events of DNA technology in which the defective gene is replaced with the functional gene. Insulin and Human growth hormone (HGH) is one of the examples of DNA technology. Tumor necrosis factor, interleukins, and interferons which are used for the treatment of cancer are all the result of DNA technology.
Agriculture:
Genetically modified crops are the product of DNA technology. These crops resistant to the plant pathogens and produce more yield than normal and commercially available. Scientists are also trying to figure out nitrogen-fixing bacteria from Rhizobium and to transfer it into the nitrogen-deficient soil for the maximum absorption of nitrogen. Transgenic plants are those in which genes from other sources incorporated into the plant cell to obtain desirable characteristics in the plant. Delaying the ripening of some fruits for the transport, insect-resistant crops are examples of DNA technology in the agricultural field.
Bioremediation:
Bioremediation is a process that involves the use of microbes for the efficient removal of contaminants from the soil. Due to DNA technology, some genes that are present in these bacteria Pseudomonas putida and Nitrosomonas europaea used and modify and insert them into the host to prevent the soil from toxins. Genetically modified bacteria are formed to eliminate petroleum products from the environment. Antibiotic-resistant genes are also the potential application of DNA technology, for this purpose marker gene is inserted into the genetically modified bacteria.
Animal Husbandry:
With the help of advanced methods of DNA technology, such genes are inserted into the cow’s genome for increased milk production. Transgenic animals are the products of DNA technology in which the desired gene of interest incorporated into the animal genome. Dolly, a cloned animal is an example of recombinant DNA technology in which the DNA of the donor is introduced into the recipient nucleus (DNA is removed from the recipient cell).
Biotechnology:
DNA technology helps in the production of genetically modified organisms, for example fluorescent zebra fish that possess the glow genes in its genome. This fish produces many colors such as green, blue, purple, orange, and yellow. This fluorescent fish is used as a pet in the indoor aquarium. Hence with the help of DNA technology, such types of genetically modified organisms are made for commercial purposes or for the benefit of mankind.
Food Industry:
DNA technology helps in the making of novel enzymes that play a significant role in food processing. Amylases and lipases are two important enzymes responsible for food manufacturing and processing. Different microbial strains have been developed for the synthesis of proteases. Similarly, fungal strains have also been modified to lessen the food spoilage. Lysozyme impregnation is an approach to increase the shelf-life of food products and to reduce the food spoilage.
Energy applications:
Different microorganisms, for example, blue-green algae favors hydrogen production and it is considered as one of the environmentally friendly sources. With the help of genetic engineering, cell-free technology, metabolic engineering, and by providing nutrient and growth condition cyanobacteria produces the hydrogen and other biofuels. By DNA technology methods, cyanobacteria are engineered to convert CO2 and other harmful chemicals.
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